Precise temperature measurements in optical traps are crucial for biological objects as they are highly sensitive to the temperature. We shift the trapped particle from the equilibrium position by moving the stage and record the position and the optical force synchronously. The absolute nature of the force detection method makes it suitable for measuring optical forces in a non-linear region of the optical trap increasing the precision to 0.2K. Moreover, the proposed method does not require a precalibration of the force detector and, therefore, can be used for temperature independent calibration.
Anatolii V. Kashchuk, Alexander B. Stilgoe, Timo A. Nieminen, and Halina H. Rubinsztein-Dunlop, "Absolute temperature measurements in optical tweezers by synchronized position and force measurement (Conference Presentation)," Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103471U (Presented at SPIE Nanoscience + Engineering: August 09, 2017; Published: 25 September 2017); https://doi.org/10.1117/12.2274659.5588453663001.
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