From Event: SPIE Optical Engineering + Applications, 2017
Observing the fast dynamics of specific molecules or targets in three-dimensional (3D) space and time inside a crowded and complex environment, such as living cells or tissues, remain one of the grand open challenges in modern science. Harmonic holography tackle this challenge by combining the 3D imaging capability of holography with the ultrafast, coherent optical contrast offered by second-harmonic radiating imaging probes (SHRIMPs). Similar to fluorescence, the second-harmonic signal emitted from SHRIMPs provides a color contrast against the uninterested background scattering, which can be efficiently suppressed by an optical filter. We review the latest developments in SHRIMPs and harmonic holography and discuss their further applications in fluidics and biofluidics.
Ye Pu and Demetri Psaltis, "Coherence-domain imaging with harmonic holography," Proc. SPIE 10380, Ultrafast Nonlinear Imaging and Spectroscopy V, 1038011 (Presented at SPIE Optical Engineering + Applications: August 08, 2017; Published: 7 September 2017); https://doi.org/10.1117/12.2275537.
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