Background and Objectives: UV Fluorescence Excitation Imaging (u-FEI) has been shown to be a simple but robust, non-invasive and non-contact method to visualize cells with a high proliferative rate. We had demonstrated the ability of u-FEI to visualize the re-epithelialization of skin wounds in an organ culture system. In this work, we investigated the potential of u-FEI for visualization of wound closure of partial and full-thickness skin wounds.
Study Design: Partial and full-thickness skin wounds were created in the tail of rats. Wounds were imaged weekly using u-FEI system operating at 295/340nm excitation/emission wavelengths, which correspond to the excitation/emission bands of the endogenous fluorophore tryptophan. Histology and immunohistology were used to determine the association between fluorescence intensity and proliferation of keratinocyte cells.
Results: Similar to human skin, the skin of a rat tail heals by re-epithelialization. Keratinocytes migrated and proliferated from the edge and skin appendages of partial-skin wounds to close the wound by creating neo-epidermis. The fluorescence intensity of the whole wound area increased uniformly during week one and decreased to non-wounded control levels around week three. For full-thickness wounds, keratinocytes migrated only from the wound edges as skin appendages were missing. The fluorescence intensity was higher by the wound edge and marched towards the center during healing. H&E and immunohistology show that changes in fluorescence intensity corresponded to newly formed epidermis.
Conclusions: u-FEI of tryptophan allowed visualization of wound closure of partial and full-thickness skin wounds in an in vivo model of wound healing by epithelialization.
Ying Wang, Antonio Ortega-Martinez, William A. Farinelli, Richard R. Anderson, and Walfre Franco, "Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)," Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670P (Presented at SPIE BiOS: January 28, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2287164.5751314178001.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for 2018 presentations, with transcripts for prior recordings added daily.
Search our growing collection of more than 16,000 conference presentations, including many plenaries and keynotes.