Congenital coronary anomalies can result in severe consequences such as arrhythmias and sudden death. However, the etiology of abnormal embryonic coronary microvasculature development is understudied. Using a novel contrast-agent-based optical coherence tomography (OCT) technique, scatter labeled imaging of microvasculature in excised tissue (SLIME), we compared diseased and normal embryonic quail coronary microvasculature in 3D. Congenital heart defects associated with fetal alcohol syndrome (FAS) were induced in a quail model by injecting 40 uL of 50% ethanol solution into eggs during gastrulation. These and saline-injected quail eggs were incubated until stage 36. SLIME contrast agent was perfused through the aortas of embryos and fixed in the vessels with a crosslinking agent. Dissected hearts were treated with a CUBIC-1 clearing agent and the scattering contrast labeled vasculature were imaged using customized spectral domain OCT systems. SLIME data revealed that coronary microvasculature of the control group was organized as parallel bundles over the left ventricle near the apex, whereas in a majority of the ethanol-treated embryos coronary microvasculature had chaotic patterns in similar regions. These differences in alignment of microvasculature have not been previously described in this disease model. Quantitative and statistical assessment will aid in evaluating the significance of this coronary defect. Future investigations will determine whether coronary mispatterning may reflect misalignment of cardiomyocytes that could lead to other negative consequences.
Meredith C. G. Broberg, Yehe Liu, Andrew M. Rollins, Michiko Watanabe, and Michael W. Jenkins, "Using optical coherence tomography to detect disturbances in coronary microvascular in a model of fetal alcohol syndrome (Conference Presentation)," Proc. SPIE 10472, Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV, 104720N (Presented at SPIE BiOS: January 28, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2290815.5751476034001.
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