In this work, we present the first histological in vivo and ex vivo study of effects of fractional Er fiber laser (wavelength 1550 nm, peak power 25 W) on keratinized gum and alveolar mucosa for gum regeneration. Biopsy with subsequent NBTC staining was used as primary evaluation technique. Ex vivo, porcine tissue model was used. Effects of pulse energy, beam diameter, and beam divergence were investigated in detail. It has been demonstrated that under optimal conditions columns up to 800 μm in depth could be reliably produced with 130 mJ pulses. Clinically, 2 subjects were treated and 4 punch biopsies were collected. The results were compared with ex vivo data. Both ex vivo and in vivo datasets suggest feasibility of a dental fractional system intended for gum regeneration.
Ksenia Shatilova, Georgii Aloian, Maria Karabut, Valentina Ryabova, Ilya V. Yaroslavsky, and Gregory Altshuler, "In vivo and ex vivo characterization of a novel Er fiber laser system for fractional treatment of soft oral tissues," Proc. SPIE 10473, Lasers in Dentistry XXIV, 104730J (Presented at SPIE BiOS: January 29, 2018; Published: 8 February 2018); https://doi.org/10.1117/12.2290734.
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