Degenerative conditions such as keratoconus and Fuch’s dystrophy can alter over time the cellular structure of the human corneal epithelium and endothelium respectively. A high-speed UHR-OCT system, capable of generating volumetric images of the cellular structure of the human cornea was built. The UHR-OCT system has a compact fiber-optic design that utilizes a commercial femtolaser with the central wavelength of 790 nm and 3dB spectral bandwidth of 150 nm to achieve ~ 1.4 µm axial resolution in corneal tissue. The optical design of the OCT imaging probe ensured ~2 µm OCT lateral resolution in corneal tissue. At the detection end of the UHR-OCT system, a high-resolution spectrometer (Cobra, Wasatch Photonics) is interfaced with a novel line scan camera. The camera has a tall pixel design, 2048 pixel array and a maximum readout rate of 250 kHz. The system’s SNR was 96 dB at 100 µm away from the zero delay line, with a 10 dB roll-off over 1.5 mm scanning range for ~800 µm power of the imaging beam incident on the corneal surface. Volumetric images of healthy and pathological corneas were acquired in-vivo from healthy volunteers and subjects with keratoconus and Fuch’s dystrophy and the images were compared with typical histological images. This study was approved by the University of Waterloo Research Ethics Committee.
Zohreh Hosseinaee, Bingyao Tan, Kirsten Carter, Denise Hileeto, Luigina Sorbara, and Kostadinka Bizheva, "In-vivo volumetric imaging of the cellular structure of healthy and pathological human cornea with high-speed UHR-OCT (Conference Presentation)," Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104740Q (Presented at SPIE BiOS: January 28, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2291216.5751469805001.
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