Purpose: To investigate the feasibility of using spectral domain optical coherence tomography (SD-OCT), infra-red and multicolor fundus imaging for monitoring the safety and efficacy of therapeutics delivery into the extravascular spaces of the choroid (EVSC).
Methods: Two hundred and fifty microliters containing Indocyanine Green (ICG), sodium fluorescein, iron oxide nanoparticles (IONPs) or 15 million human bone marrow stromal cells (hBMSCs) were injected using a novel minimally-invasive adjustable-depth blunt injector into the EVSC of New Zealand White rabbits, 3.5 mm posterior to the limbus. SD-OCT, infra-red and multicolor fundus imaging and histology analysis were performed to assess injection safety and efficacy.
Results: Infra-red wide angle (102°) imaging demonstrated that injected therapeutics covered over 80% of the posterior eye surface across the EVSC. Multicolor imaging demonstrated that injected dyes were localized in the matrix between the choroidal blood vessels. SD-OCT analysis revealed no retinal detachment, choroidal hemorrhages or inflammation up to 10 week following cell transplantation. These findings were supported by histology analysis.
Conclusions: Multimodal imaging including infra-red and multicolor fundus imaging as well as SD-OCT enables longitudinal monitoring of safety and efficacy of therapeutics delivery into the EVSC. Moreover, these imaging techniques enable to quantitatively determine the distribution of injected therapeutics in the EVSC.
Ygal Rotenstreich, Adi Tzameret, Sapir E. Kalish, Ettel Bubis, Michael Belkin, Iris Moroz, and Ifat Sher-Rosenthal, "Multimodal imaging for monitoring delivery of therapeutics into the extravascular spaces of the choroid (Conference Presentation)," Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104741F (Presented at SPIE BiOS: January 29, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2288886.5751467588001.
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