Full Field OCT (FFOCT) is a shot noise limited interferometric microscopy technique that uses incoherent light and has proved to be an effective diagnostic tool in terms of sensitivity and specificity. We have used the FFOCT setup built by LLTech for the analysis of various cancerous tissues corresponding to the following organs: breast, skin, prostate, lungs, ENT, bladder, brain etc. The scores obtained were found in the range between 80 and 98%. To do better and to provide informations that the histology does not carry we have studied, using the same setup, the temporal dependence of our signals which we found to be related to the cellular metabolism. We have used the new high speed and high full well capacity of the Adimec camera to achieve a time analysis ranging between 2 and a few thousands of ms. We thus obtain a new contrast which constitutes a biomarker at the sub-cellular scale. We monitor the characteristic frequencies and amplitude of the signal and display them on the images of the tissues using a new processing code of the time series. This metabolic contrast also reveal the evolution of the activity of cancer cells under treatments such as chemotherapy. We will illustrate this new approach through examples of cancer tissues that are planned to be used as intraoperative tools.
Albert Claude Boccara, Clement Apelian, Emilie Benoit a la Guillaume, Alban Mounier, and Eugénie Dalimier, "Static and dynamic full field OCT: an endogenous biomaker? (Conference Presentation)," Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 1047803 (Presented at SPIE BiOS: January 27, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2287522.5751476590001.
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