Monitoring of visual functioning of the retina is significant for characterizing retinal degenerative diseases. Electroretinogram is the current method for measuring the electrical responses of the retina to light. However, it requires placement of electrodes on cornea, leading to contact related uncomfortable feeling. Here, we report use of near-infrared low-coherent light for non-contact, label-free in-vivo detection of retinal activities in response to visual stimulation. We utilized phase sensitive optical coherence tomography for measuring fluctuations of light reflected from retina of wild type and retinal degenerated mice. With visual stimulation, fluctuations in optical path length difference were found to be higher than that without visual stimulation in wild type mice. However, no such changes observed in mice with photoreceptor degeneration. Our findings open up possibility for clinical use of this method for non-contact label free characterization of retinal functioning and identification of dystrophies.
Subrata Batabyal, Sivakumar Gajjeraman, Weldon Wright, and Samarendra K. Mohanty, "In-vivo label-free optical detection of neural activities in retina (Conference Presentation)," Proc. SPIE 10482, Optogenetics and Optical Manipulation 2018, 104820J (Presented at SPIE BiOS: January 27, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2291755.5751486316001.
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