The incident field size and the interplay of absorption and scattering can influence the in-vivo light fluence rate distribution and complicate the absolute quantification of fluorophore concentration in-vivo. In this study, we use Monte Carlo simulations to evaluate the effect of incident beam radius and optical properties to the fluorescence signal collected by isotropic detector placed on the tissue surface. The optical properties at the excitation and emission wavelengths are assumed to be identical. We compute correction factors to correct the fluorescence intensity for variations due to incident field size and optical properties. The correction factors are fitted to a 4-parameters empirical correction function and the changes in each parameter are compared for various beam radius over a range of physiologically relevant tissue optical properties (μa = 0.1 – 1 cm-1 , μs’= 5 – 40 cm-1 ).
Yi Hong Ong, Jarod C. Finlay, and Timothy C. Zhu, "Monte Carlo modeling of fluorescence in semi-infinite turbid media," Proc. SPIE 10492, Optical Interactions with Tissue and Cells XXIX, 104920T (Presented at SPIE BiOS: January 31, 2018; Published: 26 February 2018); https://doi.org/10.1117/12.2290137.
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Study of self-shadowing effect as a simple means to realize nanostructured thin films and layers with special attentions to birefringent obliquely deposited thin films and photo-luminescent porous silicon