Matrix metalloproteinases (MMPs) play important roles in the pathophysiology of cerebral ischemia. Here we visualized in vivo MMP activity in the transient middle cerebral artery occlusion (tMCAO) mouse model using multispectral optoacoustic imaging (MSOT) with a MMP-activatable probe. MSOT data was co-registered with structural magnetic resonance imaging (MRI) obtained at 7 T for localization of signal distribution. We demonstrated upregulated MMP signal within the focal ischemic lesion in the tMCAO mouse model using MSOT/MRI multimodal imaging. This convenient non-invasive method will allow repetitive measurement following the time course of MMP-lesion development in ischemic stroke animal model.
Ruiqing Ni, Markus Vaas, Wuwei Ren, and Jan Klohs, "Non-invasive detection of matrix-metalloproteinase activity in a mouse model of cerebral ischemia using multispectral optoacoustic tomography," Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104940O (Presented at SPIE BiOS: January 29, 2018; Published: 19 February 2018); https://doi.org/10.1117/12.2286313.
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