We discuss the use of Brillouin Light Scattering Microspectroscopy (BLSM) on Liquid Biopsies for obtaining complementary information in the diagnosis of different diseases. The viscoelastic properties of blood are known to play an important role for many processes necessary for survival including tissue perfusion, oxygen delivery and general circulation. They are understood to be dominated by the dense red blood cell suspension, with the plasma often modelled as a Newtonian fluid serving as an extracellular matrix. Much effort has been devoted to studying the mechanical properties of red blood cells, variations of which have been linked to numerous hereditary and metabolic disorders. Recent studies have shown also a non-Newtonian viscoelastic behavior of plasma. Though the biochemical composition of plasma can change at the onset of diseases, it is unclear if and how the structural-mechanical properties are affected. Here we discuss the measurement of the high-frequency viscoelastic properties of plasma from diseased blood using BLSM. BLSM utilizes the inelastic scattering of light from inherent thermal density fluctuations (acoustic phonons) to derive mechanical parameters such as the Longitudinal Storage and Loss Moduli. Since BLSM probes very fast relaxation processes it can be sensitive to structural/conformational changes of macromolecules. By mapping the variation and scaling of the storage and loss parameters also as a function of dilution and temperature we observe subtle differences between plasma from healthy and diseased samples. We discuss possible origins of these differences, and their potential for complementing liquid biopsies.
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