We introduce a label-free technique based on lens-free microscopy to perform cell counting and cell viability assay. Without the use of any labelling, the discrimination between alive and dead cells is obtained by analyzing the cells on the basis of their holographic signature. We have assessed this novel technique by comparing the obtained results in terms of viability and cell counting against automatic optical counting and trypan blue staining as reference methods. The lensfree measurements agree very well with the reference techniques up to ~30.106 cells/ml. We found a coefficient of determination R2 of 0.99 and a slope of 1.01 for the viability measurements (N=84 CHO cell samples).
C. Allier, T. Bordy, L. Hervé, O. Cioni, G. Esteban, M. Pisaneschi, and S. Morales, "Label-free cell viability assay using lens-free microscopy," Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970B (Presented at SPIE BiOS: January 29, 2018; Published: 20 February 2018); https://doi.org/10.1117/12.2289811.
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Study of self-shadowing effect as a simple means to realize nanostructured thin films and layers with special attentions to birefringent obliquely deposited thin films and photo-luminescent porous silicon