Fluorescence lifetime imaging has become a common technique in microscopy. The information contained in the fluorescence decay curve can be obtained with different methods. A major issue is that the fluorescence decay must be measured at many pixels either sequentially or in parallel, which generally results in relatively noisy curves. Another major issue is data analysis of the decay at each point of an image and the visualization of the results. Nonetheless, methods of data analysis and visualization have become relatively common and today we can analyze an image for different molecular species and molecular processes. In this talk I will discuss a method, called the phasor that was developed in my lab and that is now used in several commercial instruments. Using the phasor method applied to cell autofluorescence it has become possible to construct quantitative indices of cell metabolism and to identify molecular species characteristic to some diseases.
Enrico Gratton, "Spectroscopic signatures of cells metabolism and extracellular species using phasor-FLIM (Conference Presentation)," Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049803 (Presented at SPIE BiOS: January 28, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2295142.5751624470001.
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