This presentation describes the development of the optical macroscanner and its application for metabolic imaging of large areas of tumors in mice. The scanner allows to interrogate areas as large as 15x15mm with the lateral resolution on the order of 15 micrometers. Acquisition times range from a few seconds for low pixel numbers to
several minutes for high-resolution images. We present data for NAD(P)H imaging of tumor with genetically encoded mKate2. In addition, using macroscanner we demonstrated the possibility of visualizing caspase-3 activity using the FRET-biosensor TR23K, which is based on a pair of proteins - a red fluorescent protein as a donor and a chromoprotein as an acceptor. The in vivo assay was noninvasive and could be applied in strongly and weakly fluorescent subcutaneous xenografts in mice using the FLIM-FRET method.
Vladislav I. Shcheslavskiy, Marina Shirmanova, Victoria Zherdeva, Alena Gavrina, Varvara Dudenkova, Elena Zagaynova, Alexander Savitskiy, and Wolfgang Becker, "Macroscopic time-resolved imaging of tumor (Conference Presentation)," Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980V (Presented at SPIE BiOS: January 29, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2299317.5751623723001.
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