Two-color Stimulated Raman scattering (SRS) microscopy has shown great potential in label-free digital histology with diagnostic results similar to H&E stain. However, achieving real-time two-color SRS imaging is challenging. We have precisely engineered the pulse profiles of the Stokes beams, and fully utilized the in-phase (X) and quadrature (Y) outputs of a phase sensitive lock-in amplifier to realize simultaneous two-color SRS imaging. We have demonstrated its robustness and advantages in rapid histology, as well as real-time in vivo imaging of live animals, both in transmission and epi modes. Moreover, we have also adapted this method to other pump-probe based microscopes, such as transient absorption (TA) microscopy.
Minbiao Ji, "Dual-phase stimulated Raman scattering microscopy for simultaneous two-color imaging (Conference Presentation)," Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981N (Presented at SPIE BiOS: January 30, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2286211.5751609923001.
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