We have visualized living cells and tissues using an ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system by using a sub-nanosecond supercontinuum (SC) light source. Owing to the ultrabroadband spectral profile of the SC, we can generate multiplex CARS signals in the spectral range of 500–3800 cm−1, which covers the whole molecular fingerprint region, as well as the C-H and O-H stretching regions. Through the combination of the ultrabroadband multiplex CARS method with second harmonic generation (SHG) and third harmonic generation (THG) processes, we have successfully performed selective imaging of ciliary rootlet-composing Rootletin filaments in rat retina.
Hideaki Kano, Toshihiro Akiyama, Akihito Inoko, Tsubasa Kobayashi, Philippe Leproux, Vincent Couderc, Yuichi Kaji, and Tetsuro Oshika, "CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source," Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981P (Presented at SPIE BiOS: January 30, 2018; Published: 23 February 2018); https://doi.org/10.1117/12.2287168.
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