Single fluorescent particle tracking (SPT) is a technique often used in the biophysical sciences in order to explore the environment of soft matter [C Manzo et al, Rep Prog Phys 78, 124601 (2015); H Shen et al, Chem Rev 117, 7331–7376 (2017)]. SPT has typically been performed using widefield sample illumination and fluorescent spheres at low concentration, thus limiting the achievable tracking depth and the density of mapped particle trajectories within the sample (and hence the statistical accuracy with which particle motions can be analyzed).
In this talk, we propose to use a light sheet fluorescence microscope with photoactivatable carriers for high density SPT in thick samples with depth selectivity. In particular, we will demonstrate the usefulness of this approach for investigating the environments of heterogeneous soft materials, such as agarose gels, with improved statistical accuracy, and for providing precise depth information on the mechanical and dynamical properties of inhomogeneous soft matter. Furthermore, a discussion on the applicability of our method to probe material rheology at the nanometer-scale will be presented.
Roni Shaashoua and Alberto Bilenca, "High-density single particle tracking in thick media with depth selectivity (Conference Presentation)," Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104990Y (Presented at SPIE BiOS: January 30, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2288892.5751641146001.
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