Coded aperture imaging for 3D reconstructions have been applied in both photography and microscopy to successfully recover depth information from images captured with a patterned pupil plane. Generally, single-shot coded aperture microscopy methods require the 3D sample to be extremely sparse and with no overlapping features. Here, we extend coded aperture microscopy methods to multi-shot 3D fluorescent imaging with wave-optical forward models, enabling thicker and denser 3D samples to be faithfully reconstructed with efficient data capture via compressed sensing and advanced inverse algorithms.. We demonstrate successful reconstruction of a 500um thick brine shrimp sample. We further study the minimum required number of codes to capture full fluorescent information under wide-field illumination The experiments are carried out with a 4f system and a spatial light modulator in the Fourier (aperture) domain to display the codes.
Hsiou-Yuan Liu and Laura Waller, "3D fluorescence microscopy with multi-shot coded aperture measurements (Conference Presentation)," Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991B (Presented at SPIE BiOS: January 31, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2292478.5751641147001.
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