Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.
Baturay Özgürün, "A simple and low-cost structured illumination microscopy using a pico-projector," Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000C (Presented at SPIE BiOS: January 27, 2018; Published: 20 February 2018); https://doi.org/10.1117/12.2286790.
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