We present a new interferometric and holographic approach which allows for multiple depth multiplexing in a single acquisition, using an off-axis low-coherence interferometric imaging system. This technique enables quantitative amplitude and phase imaging of multiple slices within a thicker sample, in one single image acquisition. The method is implemented using a compact interferometric module that connects to a regular digital camera, and is useful in a wide range of applications in which multiple z slices of a sample are required simultaneously. The simultaneous acquisition is carried out by using optical multiplexing of the interferograms on the camera, whereby each slice has a distinct off axis interference fringe direction in relation to the other. We demonstrate the proposed technique for quantitative optical thickness imaging of microscopic biological samples.
Lauren Wolbromsky and Natan T. Shaked, "Multiple depth multiplexing in off-axis low-coherence interferometric imaging (Conference Presentation)," Proc. SPIE 10503, Quantitative Phase Imaging IV, 105030A (Presented at SPIE BiOS: January 28, 2018; Published: 15 March 2018); https://doi.org/10.1117/12.2286846.5752154082001.
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