Gradient Light Interference Microscopy (GLIM) is a phase-shifting technique that upgrades a conventional differential interference contrast (DIC) microscope to produce quantitative phase images. Benefiting from equally matched beams, fully open microscope condenser, as well as coherence gating due to the removal of non-interferometric terms upon phase-retrieval, GLIM has the potential to enable studies of mesoscopic structure hundreds of microns thick. Nevertheless, many such biological samples are much too big to completely transmit light, requiring the use of reflection microscopy. Here we present our early work on constructing a new reflection mode GLIM microscope designed to image thick biological structures, as well as a detailed comparison between transmitted vs reflected light modalities for a number of calibration samples.
Mikhail E. Kandel, Catherine Best-Popescu, and Gabriel Popescu, "Reflection gradient light interference microscopy (epi-GLIM) for label-free imaging of bulk specimens (Conference Presentation)," Proc. SPIE 10503, Quantitative Phase Imaging IV, 105030F (Presented at SPIE BiOS: January 29, 2018; Published: 15 March 2018); https://doi.org/10.1117/12.2294032.5752156287001.
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