The cell cycle is extensively characterized, yet there is much to learn about the decision-making process involved in cell division. Here, the optical redox ratio (ratio of NADH to FAD fluorescence intensity) of MCF10A cells was imaged every 20 minutes over 12 hours using multiphoton microscopy. Cell tracking was used to monitor individual cells over time. We found a positive correlation in the variations of the optical redox ratio with the phases of the entire cell cycle. This study reveals the novel role of redox signaling in the progression the cell cycle.
Rupsa Datta, Zhaoqi Li, Allison Lau, Matthew Vander Heiden, and Melissa C. Skala, "Cell division is coupled to the optical redox ratio (Conference Presentation)," Proc. SPIE 10504, Biophysics, Biology and Biophotonics III: the Crossroads, 1050405 (Presented at SPIE BiOS: January 28, 2018; Published: 15 March 2018); https://doi.org/10.1117/12.2289514.5752159397001.
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