We present application of holographic cytology to image red blood cells in flow. We have previously shown that optical volume, the product of surface area and integrated optical phase, is an invariant parameter which can be measured using quantitative phase imaging, regardless of cell geometry or orientation, provided that the images are digitally refocused. In these experiments, we image cells in microfluidic channels to illustrate this invariance as well as provide new insights into mechanical cell properties.
Adam Wax and Han Sang Park, "Holographic cytology for imaging red blood cells in flow (Conference Presentation)," Proc. SPIE 10504, Biophysics, Biology and Biophotonics III: the Crossroads, 105040B (Presented at SPIE BiOS: January 28, 2018; Published: 15 March 2018); https://doi.org/10.1117/12.2295830.5752163922001.
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