Stimulated Raman scattering (SRS) microscopy that is capable of both high speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology, with the integration of a rapid scanning optical delay line (RSODL), which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrated SRS spectral measurements of dimethyl sulfoxide solution at low concentrations, and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.
Minbiao Ji, "Stimulated Raman scattering microscopy and spectroscopy with a rapidly scanning optical delay line (Conference Presentation)," Proc. SPIE 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management, 105050M (Presented at SPIE BiOS: January 30, 2018; Published: 15 March 2018); https://doi.org/10.1117/12.2292948.5752179947001.
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