In this paper we investigate how the density of oligonucleotides around spherical 15 nm gold nanoparticles (AuNPs) influences their stability against degradation by DNase I. We show that a significant decrease in the number of oligonucleotides attached to the AuNP surface is directly correlated with an increase in the DNA degradation by DNase I. Our experimental observations suggest that a close packing of oligonucleotides into a 3d arrangement at the surface of AuNPs endows the probes with the necessary stability required for their use in intracellular applications.
Maria-Eleni Kyriazi and Antonios G. Kanaras, "Investigating the stability of DNA-coated gold nanoparticles ," Proc. SPIE 10507, Colloidal Nanoparticles for Biomedical Applications XIII, 105070A (Presented at SPIE BiOS: January 27, 2018; Published: 23 February 2018); https://doi.org/10.1117/12.2285833.
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