A novel approach for optical biosensing can be obtained based multiphoton induced luminescence (MIL) and its dependence on plasmonic coupling. It has been shown that the proximity of spherical AuNPs determines the generation of MIL in far-field multiphoton laser scanning microscopy (MPM). A stimuli responsive contrast mediator with high sensitivity can be created by controlling the aggregated state of AuNP. In this study we explore a system based on spherical AuNPs functionalized with β-cyclodextrin and multiple β-D-lactose units (lacto-CD-AuNP). The aim of the β- D-lactose units is to target cancer cells, based on overexpression of galectin3 (Gal-3) receptors. The results demonstrate that clustering of particles, and thereby MIL signal, was only acquired from tumor cell lines, i.e., SK-MEL-28 and A431, while not from normal keratinocytes (HEKn). Thus further studies should be undertaken to translate the concept to a preclinical setting.
Marica B. Ericson, Hanna Thomsen, Jeemol James, Vladimir Kirejev, Daniel Aili, and Antonio Vargas-Berenguel, "Exploring plasmonic coupling as a stimuli responsive contrast mechanism in multiphoton microscopy," Proc. SPIE 10509, Plasmonics in Biology and Medicine XV, 1050907 (Presented at SPIE BiOS: January 29, 2018; Published: 23 February 2018); https://doi.org/10.1117/12.2288677.
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