Laser Direct Write techniques for printing applications of living material is currently a hot topic in different biomedical and engineering fields like tissue engineering, drug delivery, biosensing, etc. specialized groups in the field have been done to find and control the ideal conditions of printability, in order to maintaining unchanged the properties of the biological transferred material.
In this work we present a comprehensive study of the printability map of two of the most used biocompatible hydrogels, Sodium Alginate and Methylcellulose. We discuss the effect of hydrogel density, laser parameters influence, etc. using a blister assisted laser writing technique. In our approach we use a thick polyamide layer for blister generation, this presents huge advantages to limit the direct laser irradiation of the living material to be transferred.
In addition the physics of blister dynamics and droplet-jet formation is discussed by means of a combined study using numerical modeling of the process fluid dynamics and high speed imaging of the transfer.
Finally a particular example of advantages of the approach from the biological point of view is discussed presenting a cell viability study of Jurkat cell printing in the conditions discussed in the study
Carlos Molpeceres, Andrés Marquez, Sara Lauzurica, Miguel Gómez-Fontela, David Muñoz, and Miguel Morales, "Laser direct writing of biocompatible hydrogels using a blister assisted approach with thick polyimide layers (Conference Presentation)," Proc. SPIE 10520, Laser-based Micro- and Nanoprocessing XII, 105200O (Presented at SPIE LASE: January 31, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2283081.5751446458001.
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