Glioma accounts for the majority of brain cancer and is the most common and aggressive human cerebral disease with low survival rates, which have received much attention on how the cancer cells can be controlled. The aim of this report is to investigate the controlling of C6 glioma cells on 3D micro/nano silicon structures with different surface energy. The silicon surface topography was formed by femtosecond laser and adjusted through changing the processing parameter. The transformation of surface energy was realized by covering a layer of organosilane with low surface tention--1H,1H,2H,2H-perfluorodecyltrichlorosilane (PFDTS). The results showed that the fewest C6 cells adhered onto hierarchical micro-mountain structures with organosilane, which exhibited the anti-cell property, while the most C6 cells adhered onto nano-grain particle structures without any modification. For the same 3D structure, the adhesion force between cells and silicon surface with various structure was weaker while lowering the surface energy. Based on the analysis of fluorescence and scanning electron microscopy images, we proposed an underlining mechanism on how C6 cell morphology and adhesion is controlled by silicon 3D structure and surface energy. In addition, the formation of arbitrary cell patterns was achieved successfully. The findings may provide a conception for the preparation of cell detector and implantable biological scaffold.
Jinglan Huo, Feng Chen, Jiale Yong, Yao Fang, Qing Yang, Hao Bian, and Xun Hou, "Controlling the morphology and adhesion of C6 glioma cell by 3D micro/nano silicon structures based on femtosecond laser ablation (Conference Presentation)," Proc. SPIE 10521, Synthesis and Photonics of Nanoscale Materials XV, 105210B (Presented at SPIE LASE: January 29, 2018; Published: 14 March 2018); https://doi.org/10.1117/12.2288305.5751465973001.
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