From Event: SPIE Commercial + Scientific Sensing and Imaging, 2018
A novel application of a highly sensitive biosensor based on long-period fiber gratings (LPFG) coated with microporous polyelectrolyte coating for Gram-negative bacteria detection was investigated. The uniform microporous coating with large surface area was fabricated with weak polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) via layer-by-layer (LbL) assembly, followed by a brief exposure to acidic aqueous solutions at pH=4. The diameter of the micropores could be adjusted in a wide range by thermal treatment and ionic etching. The effect of pore size and surface topography on bacteria adhesion was examinged. Optofluidic LPFG platform for real-time monitoring of the bacteria binding/ adhesion in a flowing condition was investigated by measuring the spectral shift in the resonance wavelength. The coated LPFG platform was further functionalized with covalently immobilized bacteria antibody for specific bacterial detection with a concentration as low as 104 CFU/ml. Comparing with the widely used biosensors based on surface plasmon resonance (SPR), no moving part or metal deposition is required in our biosensor, making it highly sensitive, accurate, compact and cost effective.
Fan Yang, Tzu-Lan Chang, Henry Du, Junfeng Liang, and Fei Tian, "Label-free bacteria detection using controllable microporous polyelectrolyte coated long-period fiber gratings (Conference Presentation)," Proc. SPIE 10662, Smart Biomedical and Physiological Sensor Technology XV, 106620J (Presented at SPIE Commercial + Scientific Sensing and Imaging: April 16, 2018; Published: 15 May 2018); https://doi.org/10.1117/12.2304843.5783309656001.
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