From Event: SPIE Commercial + Scientific Sensing and Imaging, 2018
Rice blast (Magnaporthe oryzae) is one of the most devastating rice diseases affecting yield and grain quality. Applications of Fourier-Transform Infrared (FTIR) spectroscopy to detect plant stress responses to pathogens have been widely investigated; however, assessing the difference in basal resistance (PAMP-triggered immunity; compatible reaction) vs. effector-triggered immunity (incompatible reaction) has remained largely elusive. Here, we inoculated 2-week old rice seedlings (varieties Dee Gee Woo Gen and Lemont) with rice blast isolates IC17 (compatible isolate) and IB54 (incompatible isolate). Leaf tissues were collected at 6, 24, 48, 72 hours, and 7 days after inoculation, and then the constituents were extracted with dimethyl sulfoxide for FTIR analysis. Overall, distinctive profiles of compatible and incompatible reactions compared to the controls were observed. The preliminary result suggests a potential application of FTIR spectroscopy as a means for discriminating the basal resistance and effector-triggered immunity.
Mirae Oh, Jinyoung Y. Barnaby, Hoonsoo Lee, Yulin Jia, and Moon S. Kim, "Infrared fingerprint profiles of compatible and incompatible interactions of the rice blast pathogen using FTIR spectroscopy (Conference Presentation)," Proc. SPIE 10665, Sensing for Agriculture and Food Quality and Safety X, 106650B (Presented at SPIE Commercial + Scientific Sensing and Imaging: April 18, 2018; Published: 15 May 2018); https://doi.org/10.1117/12.2307189.5783315379001.
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