Otoliths are calcium carbonate crystals located in fish ears. They play an important role in zebrafish for hearing, its sense of balance and acceleration. Many studies have been conducted to understand its structure, function but also development conditions. However the encoding in the brain as a movement sensor remains unknown. Here we developed a non-invasive system capable of manipulating one or two otoliths simultaneously in different directions to simulate movement or acceleration and sound. Our system uses optical traps created with an infra-red laser at different positions on the otoliths creating forces in chosen directions. However, as the optical traps need to go through brain tissue in a live fish, it becomes difficult to determine the exact forces applied. In this study we investigate the limits of forces determination. We will present the theory and experimental measurements of optical tweezers applied to otoliths which we mostly published in Nature Communications (doi:10.1038/s41467-017-00713-2). We will also present our latest result on brain imaging in response to artificial acceleration and sound.
Itia A. Favre-Bulle, Gilles Vanwalleghem, Alexander Stilgoe, Ethan Scott, and Halina Rubinsztein-Dunlop, "Optical trapping in zebrafish (Conference Presentation)," Proc. SPIE 10723, Optical Trapping and Optical Micromanipulation XV, 107230C (Presented at SPIE Nanoscience + Engineering: August 19, 2018; Published: 17 September 2018); https://doi.org/10.1117/12.2324377.5836018666001.
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