From Event: SPIE Organic Photonics + Electronics, 2018
Three-dimensional imaging of molecular distribution in a living cell without labeling or slicing the sample has been a dream of bio-scientists. We have made it possible with use of a gold nanoparticle which explores inside a cell for detecting intracellular molecules based on SERS mechanism [1, 2]. This can be considered as a version of plasmonic tip-enhanced Raman microscopy [3,4], but the tip (as a 50nm particle) is free in a cell. Rather than controlling the nanoparticle motion with a laser trapping technology [5,6], the motion is governed by the cell function. Simultaneous tracking of particle motion provides us a molecular map of organelle transport and lysosomal accumulation. Intracellular environment e.g. pH is also mapped by a surface-functionalized particle. Spatial resolution of this microscope is ~ 65nm (the particle size) and temporal resolution ~50ms. In the presentation, movies of particle motion and a molecular map in a cell will be shown with many other results.
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5. S. Kawata, Y. Inouye, T. Sugiura, Jpn. J. Appl. Phys. 33, 1725, 1994
6. T. Sugiura, et. al. Opt. Lett. 22, 1663, 1997
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Satoshi Kawata, "Functional nanoparticles explore a living cell for intracellular dynamics and molecular distribution (Conference Presentation)," Proc. SPIE 10740, Molecular Machines, 1074004 (Presented at SPIE Organic Photonics + Electronics: August 23, 2018; Published: 18 September 2018); https://doi.org/10.1117/12.2323777.5836544898001.