To detect small-scale changes in tissue, small sampling volumes and, therefore, short source–detector separations are required. In this case, reflectance measurements are not adequately described by the diffusion approximation. It has been shown that such subdiffusive measurements are sensitive to the phase function of tissue (the probability distribution of scattering angles). Three parameters related to the tissue phase function have been proposed to describe subdiffusive reflectance: gamma, sigma, and RpNA. For an overlapping source-detector geometry (e.g. Single Fiber Reflectance spectroscopy, or SFR), it has been shown that RpNA outperforms gamma and sigma. RpNA was derived using the assumptions that detected photons undergo only a single backscatter event in combination with an arbitrary number of forward scattering events, and that all scattering angles occur within the acceptance angle of the detector, theta_NA, where theta_NA = arcsin(NA/nsample).
We further investigated the phase function influence, by determining the distribution of scattering angles for detected photons – which we term the ‘effective phase function’. We will show that the assumption that all scattering angles occur within the acceptance angle of the detector is incorrect. Based on our results for the effective phase function, we derived a new parameter, Rpeff. We performed Monte Carlo simulations for overlapping source/detector geometries for a range of phase functions, reduced scattering coefficients, NAs, and source/detector diameters, which showed that Rpeff improves the prediction of the measured reflectance compared to gamma, sigma, and RpNA. We developed a model for an overlapping source-detector geometry incorporating Rpeff, to derive scattering and absorption properties of tissue.
Anouk L. Post, Fransien Woltjer, Steven Jacques, Dick H. J. C. M. Sterenborg, Dirk J. Faber, and Ton G. van Leeuwen, "Modeling subdiffusive scattering and absorption for an overlapping source-detector geometry, e.g. single fiber reflectance spectroscopy (SFR) (Conference Presentation)," Proc. SPIE 10876, Optical Interactions with Tissue and Cells XXX, 108760U (Presented at SPIE BiOS: February 03, 2019; Published: 4 March 2019); https://doi.org/10.1117/12.2508339.6008553264001.
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