Malignant melanoma, developing from melanocytes, is a kind of high metastatic tumor. Circulating melanoma cells, as a marker for metastasis development, are found in blood or lymphatic system at the early stage. Thus, quantitative detection of circulating melanoma cells has great significance to diagnose carcinoma and monitor tumor metastasis. In contrast to in vitro detection methods and in vivo fluorescence-based flow cytometry (IVFC), the in vivo photoacoustic flow cytometry (PAFC) utilizes melanoma cells’ predominant optical absorption in the near-infrared range over other absorbers to receive the photoacoustic (PA) signals without fluorescent dye labeling in a non-invasive way. The sensitivity of the PAFC system was verified by in vitro and in vivo experiments. Besides, we solves the technical problem that blood vessels cannot be positioned in the process of in vivo detection by designing the laser signal positioning navigation system using frequency doubling technique. PAFC provides a new tool for in vivo, label-free, and noninvasive detection of circulating tumor cells (CTCs) and has strong practicality and favorable clinical prospects.
Haiwei Wu, Quanyu Zhou, Huan Zhou, Kai Ping, Bobo Gu, Hao He, and Xunbin Wei, "Non-invasive monitoring of circulating melanoma cells by in vivo photoacoustic flow cytometry," Proc. SPIE 10879, Biophotonics and Immune Responses XIV, 108790E (Presented at SPIE BiOS: February 04, 2019; Published: 7 March 2019); https://doi.org/10.1117/12.2507807.
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