Cryopreservatives like dimethyl sulfoxide and glycerol are common agents that prevent cellular damage upon freezing of tissues or entire organisms. Although the cryopreservation capabilities of these compounds have been known empirically for years, much is unknown about the actual perfusion and distribution of the agents within cells on the microscopic scale. In this contribution, we report on studies that aim to uncover the dynamic distribution of cryopreservatives in the tissue with the aid of stimulated Raman scattering microscopy, enabling a direct and real-time view of the cellular loading and accumulation dynamics of these agents at the micrometer scale.
Richard C. Prince, Eric O. Potma, and Nancy Levinger, "Following the diffusion of cryopreservatives in tissues by stimulated Raman scattering microscopy (Conference Presentation)," Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088227 (Presented at SPIE BiOS: February 07, 2019; Published: 4 March 2019); https://doi.org/10.1117/12.2510523.6008557190001.
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