We present the first experimental demonstration of super-resolution multiphoton frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM). This is obtained through a novel microscopy technique called generalized stepwise optical saturation (GSOS). GSOS√utilizes the linear combination of M steps of raw images to improve the imaging resolution by a factor of √M . Here, a super-resolution multiphoton FD-FLIM is demonstrated on various samples, including fixed cells and biological tissues, with a custom-built two-photon FD-FLIM microscope. We demonstrate simultaneous super-resolution intensity and fluorescence lifetime images of a variety of cell cultures and ex vivo tissues. Combined with multiphoton excitation, the proposed GSOS microscopy is able to generate super-resolution FLIM images deep in scattering samples.
Yide Zhang, David Benirschke, Ola Abdalsalam, and Scott S. Howard, "Super-resolution multiphoton frequency-domain fluorescence lifetime imaging microscopy by generalized stepwise optical saturation (GSOS)," Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840C (Presented at SPIE BiOS: February 02, 2019; Published: 22 February 2019); https://doi.org/10.1117/12.2507663.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for most presentations.
Search our growing collection of more than 18,000 conference presentations, including many plenaries and keynotes.