Optical stretcher is a tool in which two counter-propagating, slightly diverging, and identical laser beams are used to trap and axially stretch microparticles in the path of light. In this work, we utilized the dual-beam optical stretcher setup to trap and stretch human embryonic kidney (HEK) cells and mammalian breast cancer (MBC) cells. Experiments were performed by exposing the HEK cells to counter-propagating laser beams for 30 seconds at powers ranging from 100 mW to 561 mW. It was observed that the percentage of cell deformation increased from 16.7% at 100 mW to 40.5% at 561 mW optical power. The MBC cells exhibited significantly higher cell stretching compared to HEK cells at the same power (80 mW). Moreover, the minimum trapping power in HEK cells was 80.5mW as compared to 65.2mW in MBC cells. This study provides useful insights into the characterization of cytoskeletal elasticity in different cell types based on non-contact optical cell stretching.
Aotuo Dong, Balaadithya Uppalapati, Md. Shariful Islam, Brandon Gibbs, Ganesan Kamatchi, Sacharia Albin, and Makarand Deo, "Non-contact trapping and stretching of biological cells using dual-beam optical stretcher on microfluidic platform," Proc. SPIE 10972, Health Monitoring of Structural and Biological Systems XIII, 109722A (Presented at SPIE Smart Structures + Nondestructive Evaluation: March 07, 2019; Published: 1 April 2019); https://doi.org/10.1117/12.2514299.
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