In this work we introduce the first step toward the development of an Infrared (IR) sensoristic platform aiming to the detection and discrimination of airborne pathogens. SARS-CoV-2 Spike glycoprotein (S) is considered as a biomarker for virus recognition. A comparative spectroscopic analysis is illustrated, studying Spike glycoprotein subunit S1 from three different viruses, MERS-CoV, SARS-CoV and SARS-CoV-2. Moreover, SARS-CoV-2 variants are also investigated. The IR characterization of their S1 secondary conformation was carried out through Attenuated Total Reflection (ATR), analyzing the Amide I absorption band. In addition, pH-dependent conformational changes of SARS-CoV-2 S1 were investigated, too

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Tiziana Mancini, Annalisa D'Arco, Marta Di Fabrizio, Rosanna Mosetti, Salvatore Macis, Giovanna Tranfo, Giancarlo Della Ventura, Augusto Marcelli, Massimo Petrarca, and Stefano Lupi, "Infrared characterization of spike protein of MERS-CoV, SARS-CoV, SARS-CoV-2 and its variants: first steps toward an optical biosensing device," Proc. SPIE 12572, Optical Sensors 2023, 125720D (Presented at SPIE Optics + Optoelectronics: April 25, 2023; Published: 31 May 2023); https://doi.org/10.1117/12.2665812.