The spatial connectivity of neural circuits and the various activity patterns they exert is what forms the brain function. How these patterns link to a certain perception or a behavior is a key question in neuroscience. Recording the activity of neural circuits while manipulating arbitrary neurons leads to answering this question. That is why acquiring a fast and reliable method of stimulation and imaging a population of neurons at a single cell resolution is of great importance. Owing to the recent advancements in calcium imaging and optogenetics, tens to hundreds of neurons in a living system can be imaged and manipulated optically. We describe the adaptation of a multi-point optical method that can be used to address the specific challenges faced in the in-vivo study of neuronal networks in the cerebral cortex. One specific challenge in the cerebral cortex is that the information flows perpendicular to the surface. Therefore, addressing multiple points in a three dimensional space simultaneously is of great interest. Using a liquid crystal spatial light modulator, the wavefront of the input laser beam is modified to produce multiple focal points at different depths of the sample for true multipoint two-photon excitation.
Samira Aghayee, Dan Winkowski, Patrick Kanold, and Wolfgang Losert, "Multi-point optical manipulation and simultaneous imaging of neural circuits through wavefront phase modulation (Presentation Recording)," Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95481P (Presented at SPIE Nanoscience + Engineering: August 12, 2015; Published: 5 October 2015); https://doi.org/10.1117/12.2191538.4519370481001.
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