Super-resolution microscopy has revolutionized fluorescence imaging providing access to length scales that are much
below the diffraction limit. The super-resolution methods have the potential for novel discoveries in biology. However,
certain technical limitations must be overcome for this potential to be fulfilled. One of the main challenges is the use of
super-resolution to study dynamic events in living cells. In addition, the ability to extract quantitative information from
the super-resolution images is confounded by the complex photophysics that the fluorescent probes exhibit during the
imaging. Here, we will review recent developments we have been implementing to overcome these challenges and
introduce new steps in automated data acquisition towards high-throughput imaging.
Joe Borbely, Jason Otterstrom, Nitin Mohan, Carlo Manzo, and Melike Lakadamyali, "Quantitative high spatiotemporal imaging of biological processes," Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95540M (Presented at SPIE Nanoscience + Engineering: August 10, 2015; Published: 26 August 2015); https://doi.org/10.1117/12.2190263.
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