Effect of hydration on the dermal collagen structure in human skin was investigated using
second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department
were procured for the study. Skin samples with subject aged between 58-90 years old were used in the
study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from
the skin samples. After the control measurement, the skin tissue was either soaked in deionized water
for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was
acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per
unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG
compared to respective control tissue, while hydration sample gave a lower backward SHG. The
collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the
packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of
multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex
Ravikant Samatham, Nicholas K. Wang, and Steven L. Jacques, "Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy," Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96890W (Presented at SPIE BiOS: February 14, 2016; Published: 29 February 2016); https://doi.org/10.1117/12.2214040.
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