Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life
threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia
have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in
mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in
established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry
out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory
blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments
were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike
symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant
differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a
potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal
conditions throughout the experiment and treatment.
Karina S. Litvinova, Shakil Ahmad, Keqing Wang, Ilya E. Rafailov, Sergei G. Sokolovski, Lin Zhang, Edik U. Rafailov, and Asif Ahmed, "A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice," Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893H (Presented at SPIE BiOS: February 14, 2016; Published: 29 February 2016); https://doi.org/10.1117/12.2213026.
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