Traumatic brain injury (TBI) is a form of brain injury caused by sudden impact on brain by an external mechanical force.
Following the damage caused at the moment of injury, TBI influences pathophysiology in the brain that takes place
within the minutes or hours involving alterations in the brain tissue morphology, cerebral blood flow (CBF), and
pressure within skull, which become important contributors to morbidity after TBI. While many studies for the TBI
pathophysiology have been investigated with brain cortex, the effect of trauma on intracranial tissues has been poorly
studied. Here, we report use of high-resolution optical microangiography (OMAG) to monitor the changes in cranial
meninges beneath the skull of mouse after TBI. TBI is induced on a brain of anesthetized mouse by thinning the skull
using a soft drill where a series of drilling exert mechanical stress on the brain through the skull, resulting in mild brain
injury. Intracranial OMAG imaging of the injured mouse brain during post-TBI phase shows interesting
pathophysiological findings in the meningeal layers such as widening of subdural space as well as vasodilation of
subarachnoid vessels. These processes are acute and reversible within hours. The results indicate potential of OMAG to
explore mechanism involved following TBI on small animals in vivo.
Woo June Choi, Wan Qin, Xiaoli Qi, and Ruikang K. Wang, "Optical microangiography enabling visualization of change in meninges after traumatic brain injury in mice in vivo," Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901I (Presented at SPIE BiOS: February 16, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2213988.
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