Infrared laser light radiation can be used to depolarize neurons and to stimulate neural activity. The absorption of
infrared radiation and heating of biological tissue is thought to be the underlying mechanism of this phenomenon
whereby local temperature increases in the plasma membrane of cells either directly influence membrane properties or
act via temperature sensitive ion channels. Action potentials are typically measured electrically in neurons with
microelectrodes, but they can also be observed using fluorescence microscopy techniques that use synthetic or
genetically encoded calcium indicators. In this work, we studied the impact of infrared laser light on neuronal calcium
signals to address the mechanism of these thermal effects. Cultured primary mouse hippocampal neurons expressing the
genetically encoded calcium indicator GCaMP6s were used in combination with the temperature sensitive fluorophore
Rhodamine B to measure calcium signals and temperature changes at the cellular level. Here we present our all-optical
strategy for studying the influence of infrared laser light on neuronal activity.
David Moreau, Claire Lefort, Sylvia M. Bardet, and Rodney P. O'Connor, "Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging," Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96901M (Presented at SPIE BiOS: February 16, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2211355.
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