There was a renewed interest, during the recent years, in the imaging and tracking of targeted cells or organelles for
a variety of biomedical and lab-on a chip applications that include particles movement. However, nonspecific
illumination during tracking can have adverse effects such as heating, reduced image contrast and photo bleaching.
In fact, current available tracking and imaging systems are unable to selectively illuminate the particle being
tracked. To fill this void, we have developed a fiber optics based probe system incorporating a spatial light
modulator (SLM) and an imaging fiber bundle for selective illumination on the targeted particle. A GRIN lens is
attached at the distal endface of the image fiber bundle for optimised illumination and collection. A tracking
algorithm is developed in order to enable controlled illumination through SLM to target the illumination point or
location in accordance with the particle movement and size variation. Further with this probe, particles can be
illuminated with light pulses of controllable duty cycle and frequency. The proposed methodology and developed
probe have good significance and expected to find potential applications areas such as optogenetics, cell signalling
studies, and lab-on a chip systems.
Anant Shinde, Sandeep Menon Perinchery, and Murukeshan Vadakke Matham, "Targeted illumination and tracking using optical fiber probe for optogenetics application," Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 969028 (Presented at SPIE BiOS: February 13, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2211282.
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