Appropriate micro-optical tools are required to exploit the key advantages of optogenetics in neuroscience, i.e. optical
stimulation and inhibition of neural tissue at high spatial as well as temporal resolutions, providing cell specificity and
the opportunity to simultaneously record electrophysiological signals. Besides the need for minimally invasive probes
mandatory for a reduced tissue damage, highly flexible or wireless interfaces are demanded for experiments with freely
behaving animals. Both these technical system requirements are achieved by integrating miniaturized waveguides for
light transmission combined with bare laser diode (LD) chips integrated directly into neural probes.
This paper describes a system concept using integrated, side emitting LD chips directly coupled to miniaturized
waveguides implemented on silicon (Si) substrates. It details the fabrication, assembly, and optical as well as electrical
characterization of waveguides (WG) made from the hybrid polymer Ormorcere. The WGs were photolithographically
patterned to have a cross-section of 20x15 μm2. Bare LD chips are flip-chip bonded to electroplated gold (Au) pads with
±5 μm accuracy relative to the WG facets. Transmitted radiant fluxes for blue (430 nm, (Al,In)GaN) and red (650 nm,
AlGaInP) LDs are measured to be 150 μW (ID = 35 mA, 5% duty cycle) and 4.35 μW (ID = 225 mA, 0.5% duty cycle),
respectively. This corresponds to an efficiency of the coupled and transmitted light of 44% for the red LDs. Long term
measurements for 24 h using these systems with red LDs showed a decrease of the radiant flux of about 4% caused by
LD aging at stable WG transmission properties. WGs immersed into Ringer’s solution showed no significant change of
their optical transmission properties after four weeks of exposure to the ionic solution.
Michael Schwaerzle, Julian Nehlich, Ulrich T. Schwarz, Oliver Paul, and Patrick Ruther, "Hybrid polymer waveguide characterization for microoptical tools with integrated laser diode chips for optogenetic applications at 430 nm and 650 nm," Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902K (Presented at SPIE BiOS: February 14, 2016; Published: 9 March 2016); https://doi.org/10.1117/12.2212554.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the conference proceedings. They include the speaker's narration along with a video recording of the presentation slides and animations. Many conference presentations also include full-text papers. Search and browse our growing collection of more than 12,000 conference presentations, including many plenary and keynote presentations.