Chorioretinal blood vessel morphology in mice is of great interest to researchers studying eye disease
mechanisms in animal models. Two leading retinal imaging modalities -- Optical Coherence
Tomography (OCT) and Scanning Laser Ophthalmoscopy (SLO) -- have offered much insight into
vascular morphology and blood flow. OCT “flow-contrast” methods have provided detailed mapping of
vascular morphology with micrometer depth resolution, while OCT Doppler methods have enabled the
measurement of local flow velocities. SLO remains indispensable in studying blood leakage,
microaneurysms, and the clearance time of contrast agents of different sizes. In this manuscript we
present results obtained with a custom OCT/SLO system applied to visualize the chorioretinal vascular
morphology of pigmented C57Bl/6J and albino nude (Nu/Nu) mice. Blood perfusion maps of choroidal
vessels and choricapillaris created by OCT and SLO are presented, along with detailed evaluation of
different OCT imaging parameters, including the use of the scattering contrast agent Intralipid. Future
applications are discussed.
Mayank Goswami, Pengfei Zhang, Edward N. Pugh, and Robert J. Zawadzki, "Visualization of chorioretinal vasculature in mice in vivo using a combined OCT/SLO imaging system
," Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96930G (Presented at SPIE BiOS: February 13, 2016; Published: 2 May 2016); https://doi.org/10.1117/12.2213609.
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