Intrinsic optical signal (IOS) imaging is a promising noninvasive method for advanced study and diagnosis of eye
diseases. Before pursuing clinical applications, more IOS studies employing animal models are necessary to establish the
relationship between IOS distortions and eye diseases. Ample mouse models are available for investigating the
relationship between IOS distortions and eye diseases. However, in vivo IOS imaging of mouse retinas is challenging
due to the small ocular lens (compared to frog eyes) and inevitable eye movements. We report here in vivo IOS imaging
of mouse retinas using a custom-designed functional OCT. The OCT system provided high resolution (3 μm) and high
speed (up to 500 frames/s) imaging of mouse retinas. An animal holder equipped with a custom designed ear bar and bite
bar was used to minimize eye movement due to breathing and heartbeats. Residual eye movement in OCT images was
further compensated by accurate image registration. Dynamic OCT imaging revealed rapid IOSs from photoreceptor
outer segments immediately (<10 ms) after the stimulation delivery, and unambiguous IOS changes were also observed
from inner retinal layers with delayed time courses compared to that of photoreceptor IOSs.
Benquan Wang and Xincheng Yao, "In vivo intrinsic optical signal imaging of mouse retinas," Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96930H (Presented at SPIE BiOS: February 13, 2016; Published: 4 March 2016); https://doi.org/10.1117/12.2212810.
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