Combination photodynamic therapy (cPDT) in which vitamin D (VD) is given prior to aminolevulinate, a precursor
(pro-drug) for protoporphyrin IX (PpIX), is an approach developed in our laboratory. We previously showed that 1α,25-
dihydroxyvitamin D3 (calcitriol), given prior to PDT, enhances accumulation of PpIX and improves cell death post-PDT
in a mouse skin cancer model. However, since calcitriol poses a risk for hypercalcemia, we replaced systemic calcitriol
with oral cholecalciferol (D3), administered as a high (tenfold, “10K”) diet over a ten-day period. Here, we ask whether
VD deficiency might alter the response to cPDT. Nude mice were fed a VD-deficient diet for at least 4 weeks
(“deficient”); controls were fed a normal 1,000 IU/kg diet (“1K”). Human A431 cells were implanted subcutaneously
and mice were switched to the 10K diet or continued on their baseline diets (controls). In other experiments, mice
received a human equivalent dose of 50,000 IU D3 by oral gavage, to simulate administration of a single, high-dose VD
pill. At various times, tumors were harvested and serum was collected to measure levels of VD metabolic intermediates.
A significant increase in PpIX levels and in the expression of differentiation and proliferation markers in tumor tissue
was observed after VD supplementation of both the deficient and 1K mice. Further results describing mechanistic details
of PpIX enhancement through alteration of heme- and VD-metabolic enzyme levels will be presented. Based on these
results, a clinical study using oral vitamin D prior to PDT for human skin cancer should be performed.
Sanjay Anand, Erik Thomas, Tayyaba Hasan, and Edward V. Maytin, "Vitamin D for combination photodynamic therapy of skin cancer in individuals with vitamin D deficiency: Insights from a preclinical study in a mouse model of squamous cell carcinoma," Proc. SPIE 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV, 96940K (Presented at SPIE BiOS: February 14, 2016; Published: 1 March 2016); https://doi.org/10.1117/12.2214463.
Conference Presentations are recordings of oral presentations given at SPIE conferences and published as part of the proceedings. They include the speaker's narration with video of the slides and animations. Most include full-text papers. Interactive, searchable transcripts and closed captioning are now available for most presentations.
Search our growing collection of more than 26,000 conference presentations, including many plenaries and keynotes.